molecular characterization and analysis of antimicrobial activity of endophytic fungi from medicinal plants in saudi arabia

background endophytic fungi, which have been reported in numerous plant species, are important components of the forest community and contribute significantly to the diversity of natural ecosystems. objectives the current study aimed to evaluate and characterize, at the molecular level, the diversity and antimicrobial activities of endophytic fungi from medicinal plants in saudi arabia. materials and methods fungi growing on plant segments were isolated and identified based on morphological and molecular characteristics. the isolates were grouped into 35 distinct operational taxonomic units, based on the sequence of the internal transcribed spacer regions in the rrna gene. the colonization frequency and the dominant fungi percentage of these endophytic fungi were calculated. a dual culture technique was adopted to investigate the antifungal activity of these endophytes. results tamarix nilotica showed the highest endophytic diversity with a relative frequency of 27.27%, followed by cressa cretica with a relative frequency of 19.27%. the most frequently isolated species was penicillium chrysogenum with an overall colonization rate of 98.57%. seven out of 35 endophytic fungi exhibited strong antifungal activity to all plant fungal pathogens tested. p. chrysogenum, fusarium oxysporum, and f. nygamai exhibited the highest inhibition against the human pathogenic bacteria staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, and klebsiella pneumoniae. aspergillus sydowii, p. chrysogenum, and eupenicillium crustaceum showed strong antimicrobial activity against enterococcus faecalis. conclusions the antimicrobial activity of these endophytic microorganisms could be exploited in biotechnology, medicine, and agriculture.